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Funded Project |
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Funding Program:
Critical and Emerging Issues |
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Project Title:
Monitoring and rapid identification of Cucurbit leaf crumple virus, an emerging threat on vegetable in Georgia |
Project Directors (PDs):
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Lead State: GA Lead Organization: University of Georgia |
| Undesignated Funding: $10,000 |
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Start Date: Jan-01-2019 End Date: Dec-31-2019 |
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Pests Involved: Cucurbit leaf crumple virus |
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Site/Commodity: Vegetable |
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Area of Emphasis: Critical & Emerging Issues |
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Summary:
Cucurbit Leaf Crumple Virus (CuLCrV) is a whitefly-transmitted cucurbit-infecting Begomovirus; a chronic threat to vegetable production in Georgia. It is able to infect most cucurbits including cucumber, muskmelon, squash, pumpkin, and watermelon, and has been reported to infect snap bean. Different weed species can also be naturally infected with this virus and thus, serve as inoculum sources besides insect vector for cucurbit crops. Although CuLCrV was identified for the first time in 2012, it was not a major issue until 2016. In 2016, the virus came back with a vengeance and causes a significant losses to vegetable production in Georgia. There is no actual estimate of losses in dollars; even at a conservative estimate, that amount is expected to be in the tens of millions of dollars. Until today, this notorious virus disease remains a severe threat to vegetables production. Typical symptoms of CuLCrV include chlorotic leaf spots and terminal buds, leaf curling and crumpling, and interveinal yellowing (McCreight et al., 2008). In addition, plants may be stunted, resulting in severe or complete yield loss. The disease symptoms are easily confused with diseases caused by other viruses such as Squash leaf curl virus (SLCV) and Squash mosaic virus (SqMV). For proper management of this devastating viral disease, we need to have a proper monitoring and an early, quick, and accurate diagnosis tool for identifying it in early stage. Traditionally, DAS-ELISA and polymerase chain reaction (PCR) methods are used for this pathogen. However, these methods are time-consuming and require access to sophisticated and bulky laboratory equipment. In particular, the complexity of the thermal cycling equipment required for PCR restricts the use of these methods mainly to well-equipped laboratories. In this project, we propose to monitor CuLCrV in different plants as well weeds host and develop a LAMP assay for specific and rapid detection of CuLCrV under laboratory and field conditions. This assay will overcome many of the limitations of traditional assays and its sensitivity can be 1,000 times higher than regular PCR (Peng et al., 2012). Testing can be carried out rapidly (often 30 min) with minimal equipment (a water bath or heated block). The new detection technique will be valuable for a fast and less tedious detection of this whitefly-transmitted virus, which will be very useful tool to monitor CuLCrV in different host plants as conveniently in the vegetable field.
Objectives: 1. To monitor the extent that Cucurbit Leaf Crumple Virus (CuLCrV) in different plants as well as weeds host and 2. To develop a LAMP assay for specific and rapid detection of CuLCrV under laboratory and field conditions. |
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