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Funded Project |
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Funding Program:
Critical and Emerging Issues |
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Project Title:
First detection of Cotton leafroll dwarf virus (CLRDV) in the US |
Project Directors (PDs):
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Lead State: AL Lead Organization: Auburn University |
| Undesignated Funding: $10,000 |
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Start Date: Jan-01-2019 End Date: Dec-31-2019 |
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Pests Involved: Cotton leafroll dwarf virus |
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Site/Commodity: Cotton |
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Area of Emphasis: Distribution, detection, and transmission of CLRDV |
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Summary:
Cotton (Gossypium hirsutum) is one of the most economically important crops in the southeast United States. Cotton blue disease (Cotton leafroll dwarf virus – CLRDV) was detected for the first time in the United States in Alabama during 2017 and its presence was confirmed during 2018. There is a need to determine the distribution of the virus, sequence the entire genome of the virus, develop testing methods to detect the virus from aphids, and develop transmission assays to characterize the mode of transmission, including acquisition, latent period, and inoculation access periods required for aphid transmission, and monitor times between infection and symptom development in cotton. This information will be used in future studies to develop a warning system for the disease, identify alternate hosts and varietal susceptibility, and develop IMP strategies for control. Funding is requested for supplies and reagents related to PCR testing to identify CLRDV and Begomoviruses.
Objectives: 1. Determine distribution of CLRDV in the southeast US and prepare samples from different areas for whole genome sequencing to compare virus strains. 2. Develop PCR methods to detect CLRDV from aphids. 3. Characterize the mode of aphid transmission. |
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