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Funded Project
Funding Program: Integrated Pest Management Competitive Grants Program
Project Title: Development Of A Molecular Detection Protocol For Ergot Spores In Cool-Season Grasses Grown For Seed
Project Directors (PDs):
Jeremiah Dung [1]
Philip B Hamm [2]
Darrin Walenta [3]
Navneet Kaur [4]
Lead State: OR

Lead Organization: Oregon State University
Undesignated Funding: $29,981
Start Date: Mar-02-2015

End Date: Feb-29-2016
Pests Involved: Claviceps purpurea
Site/Commodity: Grass seed for forage and turf
Area of Emphasis: early detection and alert
Summary: This is a Project Initiation proposal requesting $29,981 to develop a molecular detection protocol for ergot impacting grass seed production. Oregon and Washington are major producers of cool-season forage and turf grass seed. Ergot, caused by the fungus Claviceps purpurea, is an important seed replacement disease of grasses. Multiple fungicide applications are routinely applied before and during flowering, although studies based on spore trapping indicate that fungicide applications may not be needed in some years. The objectives of this project are to: 1) validate a newly developed quantitative PCR (Q-PCR) procedure using samples collected from spore traps; 2) provide an electronic Ergot Alert newsletter to inform growers and field personnel in the Columbia Basin, the Grande Ronde Valley, and Central Oregon when primary inoculum of ergot is present; and 3) assess the impact and value of the newsletter to grower and field personnel knowledge and disease management practices. The Q-PCR assay will be compared to quantification by microscopic methods using spore trap samples collected from commercial fields. An Ergot Alert newsletter will be implemented based on the alerts sent to Grande Ronde Valley growers in 2014 and expanded to audiences in other grass seed producing regions. A survey will be developed to evaluate the impact of the Ergot Alert newsletter. This project addresses the following W-IPM priorities: 1) invasive, resistant or emerging pest problems that are disrupting effective IPM programs in agriculture, natural lands or community settings; and 2) pest issues and concerns previously identified as priorities by stakeholder groups.

Objectives: The objective of this project is to: 1) validate a newly developed Q-PCR procedure using samples collected from spore traps; 2) provide an electronic Ergot Alert newsletter to inform growers and field personnel in the Columbia Basin, the Grande Ronde Valley, and Central Oregon when primary inoculum of ergot is present; and 3) assess the impact and value of the newsletter to grower and field personnel knowledge and disease management practices.


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